Microbial Ecology of Bacillus thuringiensis V. Selective Medium for Bacillus thuringiensis Vegetative Cells
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چکیده
منابع مشابه
Transformation of vegetative cells of Bacillus thuringiensis by plasmid DNA.
Plasmid DNA-mediated transformation of vegetative cells of Bacillus thuringiensis was studied with the following two plasmids: pBC16 coding for tetracycline resistance and pC194 expressing chloramphenicol resistance. A key step was the induction of competence by treatment of the bacteria with 50 mM Tris hydrochloride buffer (pH 8.9) containing 30% sucrose. Transformation frequency was strongly ...
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متن کاملDetermination of exotoxin in Bacillus thuringiensis cells.
The presence of exotoxin in Bacillus thuringiensis was demonstrated and its quantity in the cells determined. The concentration of exotoxin in the producing microorganism is approximately half the concentration of ATP. Exotoxin is produced at such a rate that the cell excretes 1/5 to 1/4 of its exotoxin content into the medium per minute.
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ژورنال
عنوان ژورنال: Applied Entomology and Zoology
سال: 1986
ISSN: 0003-6862,1347-605X
DOI: 10.1303/aez.21.210